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The My Delta Pi webstore
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For all types of hobby and technical items, please go further dowm
Microscopes for professional and hobby use
Welcome to this online presentation of quality microscopes to be used for hobby, professional applications and education works. Please click on a link to learn more or to buy. You will also find many more microscopes and optical instruments to select from by clicking at the links than those here presented.
There are also all types of microscopy accessories, like object glasses, cleaning equipment and object preparation tools.
Please scroll down to read about how to prepare an object for microscopy.
To see more microscopes, please click here or here
MICROSCOPES
- By clicking at any product link you get into as store where you can browse for
many more products
To find more advanced microscope models for scientific and hobby use - please click here
OTHER OPTICAL INSTRUMENTS FOR HOBBY USE AND THE PROFESSIONAL SCIENTIST
To find telescopes for hobby use and the professional scientist,
please click here.
To find binoculars and monoculars for day and night vision, binocular and
monocular cameras for day and night vision,
please click here.
HOBBY AND PROFESSIONAL EQUIPMENT OF MANY KINDS
General store for hobby products of all kinds - and many professional grade electronic products
By clicing at this banner you will find many helicopter and aircraft models, and also a lot of other articles, for example: Hobby chemistry equipment, hobby electronic equipment, professional electronic components, car models, boat models and metal detectors for hobby and professional use.
A very comprehensive store of RC models of all types
The greatest online shop of
helicopter models
Every RC aviation entousiast
simply must visit this special collection of remote control helicopter models.
You will find the most advanced helicopter models available, but also cheaper
models for the beginner can be found in this collection. You find powerful
models for though outside environments and small models that you can
use inside your own house.
Racing model cars to be used on rough pists
This is a great store of nitro driven aircraft models.
Comodities to use in your car - also a lot of tools to use on cars and on any other work
By clicking at this banner, you will find a huge collection of commodities for your car or to bring with you on your journeys, like electronic equipment, luggage bags, security items, navigation units, workshop tools and a lot more. Also technical equipment.
Design your own clothes to perfect your personal style - with Spreadshirt Designer -
Here you can decide patterns, colors, figures and texts for your own clothes by an online interface, and order the clothes you have designed. The shop will send the clothes in 24-48 hours. You can design T-shirts, men's and ladies's longsleeves, jackets, special ladie's wear, trigema, football shirts, shorts, socks, junior's clothes, headwear, underwear and several types of apparel accessories.
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How to prepare an object for microscopy
In order to look at something throug a microscope, the object must confirm to certain criteria:
- The object must be so that the details you want to see can be illuminated.
- The object must have properties so that one can focuse sharply at it
- An object must have a size so that it fits under the microscope
- The objective must be protected from contamination form the bobject
- If the object shall be used many times, it must be prepared so that it can be stored
Preparing a fresh liquid object
If you want to look at something liquid or floating in its natural shape, for example a drop of water with microorganisms and algae in it, the preparation can be very simple.
You just take a drop of the liquid onto the object glass and lay a cover glass over it. Then you can look at it right away.
If the liquid have some cruder ingredients in it, you can use an object glass with a dtch or concavity in it.
Looking at a fresh solid object.
A fresh solid object is usually too bulky to be looked at with illumination from below. If the object is absolutely still and small enough to fit under the objective, you can often look at it with illumination from above. If such an arrangement is not possible, you must make slices of it to be placed at a glass plate.
A practical thickness for the slice is ca 0.25 - 2.0 mm thick. The greater the magnification, the thinner must the slice be.
With some training you can make such slices with a sharp thin knife, scalpel or with an old fashion razor blade. However there are also instruments called microtomes to be bought to make such slices. These can be found from simple hand held devices to advanced scientific microtomes.
Then you lay the sliced object on the glass and lay the cover glass over it.
Preparing a permanent object from a liquid blending
First of all you blend some color solution in the liquid. There are colors that adhere to specific structures such as cell kernels or the cytoplasma of microorganisms. Wait for some time.
Do not use so much color that the blending get sharply colored.
You then take a drop of the colored liquid on the object glass.
The you gently warm and dry the drop.
Then lay a drop of gluing balsam on a cover glass. Such gluing balsams are to be bought. A blending of canada balsam ( a sort of natural resin ) and xylene is a commonly used glue. The blending itself is often called just Canada balsam.
Then lay the cover glass over the object with the gluing balsam towards the obejct.
You let the balsama dry, and the object is ready.
Preparing a permanent object from some solid material.
This is the most extensive procedure.
First you must cut a thin slice of the object.
Then lay the object on the object glass.Press it gently, but fermly to the glass. Usully the object will glue to the glass by itself.
Then set the glass with the object in a watery color solution for a couple of hours. If the object shall be colored, the color must be soluted into the water. If you use two colors, it is most practical to use two concecutive solutions, each with its own color.
Move the object over to a solution of alcohol and water, and let it stand there for a couple of hours.
Then move it over to a solution of pure alcohol and let it stand a couple of hours.
Then move it over to some liquid that is not soluble in water and let it stand a couple of hours. Usually xyelene is used, but toluene is also useful. These are toxic substances, so you must have some air ventilation.
At last lay a cover glass over the object with a drop of gluing balsam, and let the ready made object dry.
Use of colors for microscopy
There are a variety of color substances to choose from when preparing a preparate for microscopy. Two substances are however standardized for most applications, especially in medicine and pathology.
one of these is Eosine. This substance clor cytoplasmic structures and the collagen proteins in muscle fibres red.
The other is Methylene blue which colors DNA and RNA and hence the cell kernel.
The color Methyl blue which is not to be confused with methylene blue, can be used to color collagen fibres in muscles.
Fuchsine is a color frequently used to stain bacteria. Another stain frequently used for bacteria is Gentian violet. (Methyl violet, Crystal violet). It is used in the Gram's method of bactereal classification.
Bacteria that have a thick peptidoglycan layer around them are stained brightly blue-violet by gentiana violet. Peptido-glycan is a molecular network consisting of carbomydrate units chemically bound to each other. Other bacteria are only stained faintly pink. Those strongly stained are called Gram-positive and those others Gram-negative.